1. When you analyzed the results of your gel, did any of your experimental samples contain dyes that did
not match the four reference dyes?
All of the sample dyes corresponded to a reference dye, however, we found that yellow 5 was not found in any of the sample dyes.
2. Look at the structures of the dyes pictured here. Which of these dyes would migrate similarly to the dyes you examined in this lab? Why?
I think blue 1, beetroot red, and carminic acid would travel at similar rates because they are similar sizes. Citrus red 2 and fast green FCF would go at similar paces to the rest of the dyes.
3. Many popular dry dog foods and dog treats have FD&C dyes among their ingredients. For example, Beneful dry food contains Yellow 5, Red 40, Yellow 6, and Blue 2, and Snausages Breakfast Bites contain Red 40 lake, Yellow 6 lake, and Yellow 5 lake. (Lake dyes are the insoluble forms of the FD&C dyes.) Why do dog food manufacturers put artificial food colors in dog food?
Dyes are put in dog food to make the dogs want the food more because it looks more appealing. This also makes the dogs want to eat more dog food than if it wasn't colored.
4. Are there any reasons why artificial food colors might be preferable to natural food colors?
Color attracts all species; similarly, color makes food look more appealing, tricking us into actually thinking it is better.
5. What two factors control the distance the colored dye solutions migrate?
The size of the colored solution's molecules and its pH.
6. What force helps move the dyes through the gel?
An electrical current draws the negative charged dyes towards the positive electrode.
7. What component of the electrophoresis system causes the molecules to separate by size?
The pores of the gel allow small molecules to move easily through it. Larger molecules go slower because they can't move through the pores so easily.
Explain 8. Agarose electrophoresis is commonly used to separate molecules of DNA. Explain how you expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate.
The molecular weight of 600 daltons will move the farthest. The molecular weight of 5000 daltons will be the slowest. The smallest molecular weight will move accordingly based on size, the smaller the faster.
All of the sample dyes corresponded to a reference dye, however, we found that yellow 5 was not found in any of the sample dyes.
2. Look at the structures of the dyes pictured here. Which of these dyes would migrate similarly to the dyes you examined in this lab? Why?
I think blue 1, beetroot red, and carminic acid would travel at similar rates because they are similar sizes. Citrus red 2 and fast green FCF would go at similar paces to the rest of the dyes.
3. Many popular dry dog foods and dog treats have FD&C dyes among their ingredients. For example, Beneful dry food contains Yellow 5, Red 40, Yellow 6, and Blue 2, and Snausages Breakfast Bites contain Red 40 lake, Yellow 6 lake, and Yellow 5 lake. (Lake dyes are the insoluble forms of the FD&C dyes.) Why do dog food manufacturers put artificial food colors in dog food?
Dyes are put in dog food to make the dogs want the food more because it looks more appealing. This also makes the dogs want to eat more dog food than if it wasn't colored.
4. Are there any reasons why artificial food colors might be preferable to natural food colors?
Color attracts all species; similarly, color makes food look more appealing, tricking us into actually thinking it is better.
5. What two factors control the distance the colored dye solutions migrate?
The size of the colored solution's molecules and its pH.
6. What force helps move the dyes through the gel?
An electrical current draws the negative charged dyes towards the positive electrode.
7. What component of the electrophoresis system causes the molecules to separate by size?
The pores of the gel allow small molecules to move easily through it. Larger molecules go slower because they can't move through the pores so easily.
Explain 8. Agarose electrophoresis is commonly used to separate molecules of DNA. Explain how you expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate.
The molecular weight of 600 daltons will move the farthest. The molecular weight of 5000 daltons will be the slowest. The smallest molecular weight will move accordingly based on size, the smaller the faster.
Dyes from gel electrophoresis lab
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